Wang, PoHao , Sekhon, Rajandeep , Chopra, Surinder .
Profiling of copy specific expression patterns of a multicopy Myb gene of maize.
The maize p1 gene encodes a Myb-homologous transcription activator which regulates accumulation of phlobaphenes or 3-deoxy flavonoid pigments in floral organs. The p1 gene specified phenotypes can be readily observed in the kernel pericarp and cob glumes of mature maize ears. Alleles of p1 gene have been named according to pericarp and cob glumes pigmentation pattern. For example, the P1-rr shows red pericarp and red cob and P1-wr exhibits white pericarp and red cob. These two alleles show 99.9% sequence similarity in their coding regions. The P1-wr allele has six or more p1 gene copies while P1-rr only has one copy. The objective of this study is to identify distinct copies of P1-wr that may be expressed in a tissue specific fashion. Reverse transcription-PCR was conducted to amplify P1-wr cDNA products from pericarp and cob glumes for sequence comparisons among multiple copies. Our results show that sequence polymorphisms among different copies are revealed at positions within exon1, exon2 and exon3. Expression profiling shows copy-type pattern in certain tissues. To further study the frequency of expression among multiple copies, a large set of cDNA clones are being isolated and sequenced. Our results indicate that certain P1-wr copies are expressed more frequently than the others.
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1 - Pennsylvania State University, Department of Crop and Soil Sciences; Intercollege Graduate Degree Program in Plant Biology, 116 ASI Bldg., University Park, State College, PA, 16802
pericap color1 gene.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM