Yu, Xiaomin , Jin, Yuqin , Nikolau, Basil , Wurtele, Eve .
Expression of branched chain fatty acid genes in Arabidopsis.
Vegetable oils are composed of linear fatty acids. Although these oils provide desirable lubricant properties, the suboptimal oxidative stability at high temperatures and high pour point temperature of vegetable oils limit their applications. Esters of branched chain fatty acids can substantially improve these qualities. Either of two Bacillus genes (yhfB and yjaX), homologs of the E.coli and Arabidopsis KASIII enzymes, can confer branched chain fatty acid biosynthesis in E.coli, which does not normally produce these fatty acids. Our hypothesis is that introduction of yhfB and/or yjaX might alter fatty acid composition by inducing branched chain fatty acid biosynthesis in Arabidopsis. Both non-targeted and plastid-targeted expression of these two genes was conducted. Our results show that each of these two proteins accumulates in transgenic plants. GC-MS is being used to determine whether branched chain fatty acids or other novel compounds accumulate in these transgenic lines.
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1 - Iowa State University, Genetics, Development and Cell Biology, 433 Bessey Hall, Ames, IA, 50011, USA
2 - Iowa State University, Biochemistry, Biophysics, and Molecular Biology
3 - Iowa State University, Genetics, Development and Cell Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM