Evolution of Development and Physiology
Williams, Ashantye S , Soliman, Khairy , Michler, Charles H , Aldrich, Preston , Kantety, Ramesh .
Assessment of Genetic Diversity of Red Oak Species using DNA marker profiling.
Red oaks of the Cumberland Plateau, specifically northern red oak (Quercus rubra) southern red oak (Q. falcata), scarlet oak (Q. coccinea), black oak (Q. velutina), and shumard’s oak (Q. shumardii) are important keystone species in the maintenance of biodiversity. The last four species are capable of extensive hybridization with northern red oak, as well as with each other oak species. Identification of pure species vs. hybrids is an important attribute for understanding the population dynamics of oaks and for the economic reasons as well. Therefore, categorization of genetic diversity within and among the oak species with the use of molecular markers may facilitate unambiguous identification of trees. The objective of this study was to establish a panel of microsatellite markers (SSRs) for screening red oak populations of the Southern Cumberland Plateau. We examined the cross-species amplification of 30 SSRs developed based on northern red oak genome in other members of the family Fagaceae. We isolated DNA from cambial tissue of three trees each of northern red oak, southern red oak, scarlet oak, black oak and shumard’s oak. A majority of the SSRs exhibited cross-species amplification and thus have the potential for use in detecting molecular phylogeography of the red oaks. These markers will be used to help in establishing the genetic diversity of Quercus spp. in the Southern Cumberland Plateau.
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1 - Alabama A&M University, Plant and Soil Science, 4900 Meridian Street, Normal, AL, 35762, USA
2 - Alabama A&M University, Plant and Soil Science
3 - USDA Forest Service/Purdue University, Hardwood Tree Impovement and Regeneration Center
4 - Benedictine University, Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM