Growth and Vegetative Development
Bolton, James J , Soliman, Khairy , Wu, Zhengdao , Wilkins, Thea , Jenkins, Johnie .
Molecular Identification and Characterization of Genes Related to Cotton Fiber Initiation and Development Using the Cotton Mutant, Ligon Lintless-1 (Li-1).
Cotton mutants have been used to examine fiber gene expression and regulation. Genetic analyses of one of these mutants, Li-1, utilized in this study revealed that it is a simply inherited, monogenic dominant trait characterized by short lint fiber (~2 mm long). The objectives of this research were to identify fiber genes and to evaluate the role of the Li-1 mutant in fiber initiation and development. To accomplish these objectives, plants were grown in the greenhouse and DNA was extracted from the young leaves of Pima 3-79, the Li-1 mutant, F1 and F2 plants. Touchdown PCR was performed on DNA samples. A total of 25 SSR MapPair primers known for their high degree of polymorphism were used to screen the parents, F1s and F2s. The preliminary results showed that 22 primers consistently gave amplified products. The PCR products were confirmed with ABI 3100 Genetic Analyzer using fluorescently labeled primers. Several polymorphic bands were observed in Pima 3-79 and the Li-1 mutant parent. The sizes ranged from 83-254 bp. The F1 amplicons showed both bands expressed in the parents, confirming the true nature of the markers. All amplified products were then cloned and sequenced in search of SNPs. Furthermore, high quality RNA was isolated from the ovules/fiber of both Pima 3-79 and Li-1 for expression studies. Oligonucleotide microarray analysis is currently underway to monitor gene expression of the fiber of the Li-1 mutant at different developmental stages ranging from -3dpa up to 25dpa. Full analysis of the results will be presented.
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1 - Alabama A&M University, Plant and Soil Science, 4900 Meridian Street, Normal, AL, 35762, USA
2 - Alabama A&M University, Plant and Soil Science
3 - Texas Tech University, Plant Science
4 - USDA, Crop Science
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM