Klink, Vincent , Overall, Christopher , MacDonald, Margaret , Matthews, Benjamin .
Laser capture microdissection (LCM) and microarray analysis of the formation of the syncytium made by Heterodera glycines in roots of Glycine max.
The major pathogen of Glycine max (soybean) is the parasitic nematode Heterodera glycines (soybean cyst nematode), responsible for ~one billion dollars in lost revenue annually in the United States. H. glycines infects G. max roots and establishes a feeding site (syncytium). H. glycines derives its nourishment from the syncytium during the course of its lifecycle. We have used LCM to study gene expression during the formation of the syncytium. Prior to these studies, we performed a time-course microarray analysis of whole infected roots during both a resistant (incompatible [I]) and susceptible (compatible ([C]) reaction so that we could compare events occurring within whole roots with the syncytium. A whole root time-course microarray analysis revealed many differences in temporal events in gene expression between I and C. Gene sets unique to I and C were also identified. LCM was used to isolate syncytia from different time-points during infection. Microarray analysis of those syncytia identified numerous genes expressed at different time-points and genes unique to the early formation of syncytia in I and C. Many of these genes identified here may be useful for engineering resistance in G. max to H. glycines.
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1 - United States Department of Agriculture, 10300 Baltimore Ave., Beltsville, MD, 20705, USA
2 - George Mason University, Department of Bioinformatics and Computational Biology
3 - United States Department of Agriculture
laser capture microdissection.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM