Kakani, Aparna , Chitteti, , Brahmananda Reddy , Hong, Waifoong , Peng, Zhaohua .
Developing a method for quantitative comparison of molecular reporters among individual cells and determining the auxin level required for cell dedifferentiation in Arabidopsis.
Auxin regulates both cell differentiation and dedifferentiation in plants. It is still unknown how auxin controls these two opposite biological processes precisely in the cell. In this report, cell dedifferentiation mutant screening and characterization as well as chemical inhibition studies show that disruption of auxin efflux promotes callus formation and results in high auxin accumulation in the callus producing tissues in Arabidopsis. To determine the auxin level required for cell dedifferentiation and callus production, we have developed a method to quantify auxin levels revealed by the DR5:GUS reporter system in individual cells. Comparison of the cellular auxin levels among callus producing cells and root apical meristem cells indicates that the cellular auxin levels in callus producing cells are always higher than in the apical meristem cells. This result suggests that plant cells use auxin levels higher than meristem cells to regulate the cell dedifferentiation process and auxin levels equivalent or lower than the meristem cells to regulate other cellular activities such as cell differentiation. Molecular reporters, particularly the GUS and fluorescence proteins, have been widely used in the studies of current biological sciences. The quantification approach we use here may have broad applications in improving the accuracy of our understandings
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1 - MSU, Biochemistry and Molecular Biology
2 - Mississippi State University, Biochemistry and Molecular Biology
3 - Stering college, zoolgy
4 - Mississippi State University, Biochemistry and Molecular Biology, box 9650,Dorman Hall,, Mississippi State, MS, 39762, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM