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Large Scale Technologies and Resources

Yu, Su-May [1], Ko, Swee-Suak [2], Hong, Chwan-Yang [3], Sun, Hsou-Jan [4], Ho, Tuan-hua David [4].

Global Functional Analyses of Rice Promoters by Genomics approaches.

Promoters play key roles in conferring temporal, spatial, developmental or environmental regulation of gene expression, and they are useful for manipulating foreign gene expression in plants for both basic and applied research. Previously we have generated a large T-DNA tagged rice mutant library (TRIM), in which the T-DNA was designed with a gene/promoter trap system, by placing a promoter-less GUS gene next to the right border of T-DNA. GUS activity screens of this library offer in situ and in planta identifications and analyses of promoter activities in their native configurations in the rice genome. In the present study, we systematically performed GUS activity screens of the rice mutant library for genes/promoters constitutively, differentially or specifically active in vegetative and reproductive tissues. More than 8200 lines have been screened, and 11 and 22% of them displayed GUS staining in vegetative tissues and in flowers, respectively. Among the vegetative tissue active promoters, the ratio of leaf active vs. root active is about 1.6. Interestingly, all the flower active promoters are anther active, but with varied activities in other flower tissues. To identify tissue specific ABA/stress up-regulated promoters, we compared microarray data of ABA/stress induced genes with those of tissue-specific expression determined by promoter trap GUS staining. Following this approach, we showed that the peroxidase 1 gene promoter was ABA up-regulated by 4 fold within one day of exposure to ABA and its expression is lateral root specific. We suggest that this be an easy bioinformatics approach in identifying tissue/cell type specific promoters that are up-regulated by hormones or other factors.


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1 - Academia Sinica, Institute of Molecular Biology, Nankang, Taipei, 115, Taiwan, ROC
2 - Academia Sinica, Biotechnology Experimental Center in Southern Taiwan
3 - National Taiwan University, Department of Agricultural Biochemistry
4 - Academia Sinica, Institute of Plant and Microbial Biology

Keywords:
Promoters
Functional genomics
bioinformatics
rice
Promoter trap
Biotechnology.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P42003
Abstract ID:407


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