Jikumaru, Yusuke , Hanada, Atsushi , Sekimoto, Masayo , Nambara, Eiji , Yamaguchi, Shinjiro , Kamiya, Yuji .
Hormonome Analysis at RIKEN Plant Science Center.
Plant hormones interact with each other and regulate many aspects of plant growth and development. Therefore it is important to analyze all the hormones from the same plant materials. We used to use GC-MS for hormone analysis. However, this method is not necessarily suitable for high-throughput and comprehensive hormone analysis. We have recently introduced an LC-ESI-MS/MS system, which does not require derivatization of hormones. Using both GC-MS and LC-MS we have performed comprehensive hormone analysis. We could identify 16 different GAs by LC-MS from Arabidopsis. (Varbanova M. et al. Plant Cell 2007 Jan.) ABA and PA, DPA and ABA-GE were analyzed from submerged rice shoots (Saika H. et al. Plant Cell Physiol. 2007 published on-line). In a special case, we could quantify ABA even from a single seed of Arabidopsis cyp707a1/a2/a3. Analysis of cytokinins by LC-MS has already been established in RIKEN PSC. We are currently analyzing auxins, jasmonic acid and salicylic acid. Although brassinosteroids are still difficult to be analyzed by LC-MS at a high sensitivity, our new LC-MS system allows us to quantify major plant hormones using internal standards. We will also report the present situation of the hormone analysis platform of RIKEN PSC.
Log in to add this item to your schedule
1 - RIKEN, Plant Science Center
2 - RIKEN, Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM