Growth and Vegetative Development
Che, Ping , Lall, Sonia , Howell, S.H .
Developmental steps in acquiring competence for shoot regeneration.
Arabidopsis shoots regenerate from root explants in tissue culture through a two-step process requiring preincubation on an auxin-rich callus induction medium (CIM) followed by incubation on a cytokinin-rich shoot induction medium (SIM). During CIM preincubation, root explants acquire competence to respond to shoot induction signals. During CIM preincubation, pericycle cells in root explants undergo cell divisions and dedifferentiate, losing the expression of a pericycle cell-specific marker. These cells acquire competence to form green callus after only one day CIM preincubation and to form shoots after 2-3 days CIM preincubation. Reversible DNA synthesis inhibitors and auxin response mutants (axr1 and axr4) interfered with the acquisition of competence to form shoots. Genes requiring CIM preincubation for upregulation on SIM were identified by microarray analysis and included RESPONSE REGULATOR 15 (ARR15), POLYGALACTURONASE INHIBITING PROTEIN 2 (PGIP2) and WUSCHEL (WUS). These genes served as developmental markers for the acquisition of competence because the CIM preincubation requirements for ARR15 and PGIP2 upregulation correlated well with the acquisition of competence to form green callus, and the CIM preincubation requirements for WUS upregulation matched those for shoot formation. Unlike ARR15, another cytokinin inducible, A-type ARR gene, ARR5, was upregulated on SIM, but the induction did not require CIM preincubation. These findings indicate that competencies for various events associated with shoot regeneration are acquired progressively during CIM preincubation, and that certain genes, normally upregulated on SIM are under forms of repressive control, which can be relieved by CIM preincubation.
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1 - Iowa State University, Plant Sciences Institute
2 - Iowa State University, Plant Sciences Institute, 1073 Roy J. Carver Co-Laboratory, Iowa State University, Ames, Iowa, 50011, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM