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Abstract Detail

Temperature Responses

Bennett, Cassandra [1], Pacheco, Arlene [2], del Rio, Sonia [1], Ortiz, Carlos [1], Louzada, Eliezer [3].

Citrus Low Temperature Responsive Gene (Clt): Genome Organization and Expression Analysis.

A citrus low temperature responsive gene called Clt, previously isolated from the cold hardy citrus relative Poncirus trifoliata (PT), shows a peculiar expression pattern when the plant is cold acclimated. The expression starts at 10°C and it increases as the temperature drops. As the plant is maintained at low temperature for longer than 36 h, other transcripts are expressed. Control, non-acclimated plants, expresses constitutively another gene with similar open reading frame (ORF) of Clt, however, it lacks a 98 nucleotide at the 3 untranslated region (3UTR). Sequencing analysis for all transcripts induced by low temperature shows an interesting pattern of intron retention. Comparison of expression of Clt in the cold hard PT and in a cold sensitive citrus species demonstrated a time response fashion with the cold sensitive showing very reduced expression even after seven days of acclimation. The Clt-like gene from the cold sensitive showed an additional four amino acids at the beginning of the ORF, with the remaining nucleotides being the same as in the Clt ORF. Differences at the promoter area were also observed in the cold sensitive species. A detailed genomic analysis and additional expression pattern will be provided.

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1 - Texas A&M University Kingsville, Citrus Center
2 - Texas A&M University Kingsville, Texas A&M University Kingsville
3 - Texas A&M University Kingsville, Citrus Center, 312 N. International Blvd, Weslaco, TX, 78596, USA

freezing tolerance.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P08010
Abstract ID:354

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