| Abstract Detail
Mechanisms of Gene Regulation Wright, Janet D [1], Schoenbeck, Mark [2]. Experimental comparison of pre-mRNA splicing between flowering plant lineages. Plant intron splicing is distinct in that plant introns generally are comparatively short uridine-rich sequences lacking either a conserved branchpoint, as in yeast introns or a pyrimidine tract, as in mammalian introns. Predictions have been made as to differences in intron processing between dicots such as Arabidopsis and monocots such as maize based upon computer modeling using large quantities of genomic information. Experimental studies employing sequence manipulation of either synthetic introns or a relatively limited number of functional introns suggest monocots tend to be less restrictive in intron identification and processing than dicots. Our system tested the capacity of a range of plants to splice functional introns from divergent sources in a common molecular substrate. The potato cytoplasmic GAPDH cDNA was engineered to accept corresponding introns with their flanking sequences from the GAPDH gene of Arabidopsis thaliana, Medicago sativa, and Zea mays. Additionally, the second intron from the Amborella trichopoda AmAP3 gene with its flanking sequences was also tested in this substrate. Processed transcripts were then recovered from Allium tuberosum, Petunia hybrida, Magnolia soulangiana, and Raphanus sativus. Unspliced transcripts were recovered in every system except Medicago from Petunia. Spliced Arabidopsis transcripts were recovered from Petunia. Spliced Medicago transcripts were recovered from Petunia and Raphanus. Spliced maize transcripts were recovered from Petunia and Magnolia. Spliced Amborella transcripts were recovered only from Magnolia. No mis-spliced transcripts were recovered. These observations may indicate a divergence in splicing specificity since the divergence of monocots and eudicots from basal angiosperms.
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1 - University of Nebraska at Omaha, Biology, Allwine Hall 114, 60th and Dodge Sts., Omaha, Nebraska, 68182-0040, United States
2 - University of Nebraska at Omaha, Biology
Keywords:
gene regulation
intron splicing
intron identification.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P36006
Abstract ID:351 |
