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Abstract Detail


Organelle Biology

Yang, Pingfang [4], Reumann, Sigrun [1], Shrestha, Kalpana [4], Kyaw, Aung [4], Olsen, Laura [2], Weber, Andreas [3], Hu, Jianping [4].

Using systems biology to understand peroxisomal proteomes in Arabidopsis.

Plant peroxisomes are essential players in various physiological and metabolic pathways in development and during stress responses. However, the protein composition and metabolic and regulatory networks associated with peroxisomes is still poorly understood. Using a combinatorial approach including proteomics, bioinformatics, cell biology, and reverse genetics, we are cataloging the membrane and matrix proteomes of three major peroxisomal variants: leaf peroxisomes from green leaves, glyoxysomes from germinating seedlings, and gerontosomes from senescent tissue. To date we have used mass spectrometry-based shotgun proteomics to identify novel membrane and matrix proteins from leaf peroxisomes; analysis of other peroxisomal subtypes are underway and protein complexes will be identified in the future. Several dozen novel candidate peroxisomal proteins have been identified by this approach. A subset of the novel proteins identified by both proteomic and bioinformatic analyses were subjected to subcelluar localization study using fluorescence microscopy of GFP-fusion proteins; many have been confirmed to be peroxisomal. Based on results from a series of physiological and biochemical assays conducted with sequence-indexed T-DNA insertion mutants of some of these novel genes, hypotheses will be generated regarding their functions in peroxisomes. In addition to providing the scientific community with a much-needed comprehensive inventory of plant peroxisomal proteins, we will also disseminate a set of tagged reporter constructs for follow-up studies. This work is funded by the NSF Arabidopsis 2010 program.


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Related Links:
The Arabidopsis 2010 project on peroxisomal networks


1 - University of Göttingen, Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences
2 - University of Michigan, Department of Molecular, Cellular, and Developmental Biology
3 - Michigan State University, Plant Biology Department
4 - Michigan State University, MSU-DOE Plant Research Laboratory, East Lansing, MI, 48824, USA

Keywords:
peroxisomal proteome
proteomics
subcellular targeting
reverse genetics
Arabidopsis.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P18011
Abstract ID:350


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