Unable to connect to database - 04:53:56 Unable to connect to database - 04:53:56 SQL Statement is null or not a SELECT - 04:53:56 SQL Statement is null or not a DELETE - 04:53:56 Botany & Plant Biology 2007 - Abstract Search
Unable to connect to database - 04:53:56 Unable to connect to database - 04:53:56 SQL Statement is null or not a SELECT - 04:53:57

Abstract Detail

Reproductive Development

Wijeratne, Asela [1], Sun, Yujin [2], Zhang, Wei [2], Liu, Wenlei [3], Ma, Hong [4].

Genome-wide expression analysis of developing wild-type and mutant Arabidopsis anthers: establishing a regulatory network for anther development.

The angiosperm anther contains specialized reproductive and somatic cells that are required for pollen development. Although several genes have been found to be important for anther cell division and differentiation, the effect of these genes on anther gene expression and their relationships are not clear. To gain further insights into the differentiation of microsporocytes and tapetum and to investigate gene interactions, we have performed microarray expression profile of the wild-type anther and compared that with those of male sterile mutant anthers near the time of meiosis. The significance analysis of microarray (SAM) method was used to identify genes with statistically significant changes among the genotypes and several hundred genes were differentially expressed in each of the mutant anthers compared with the wild-type anthers. These genes were grouped into different co-expression clusters using the K-mean clustering method. A number of known genes critical for tapetum development or meiosis were found in several clusters, suggesting that these clusters contain additional genes involved in these processes. To obtain clues about possible co-regulation within co-expression clusters, we searched for enriched cis-regulatory motifs in corresponding putative promoter regions. The information obtained from these analyses, together with the published literature, was then used to develop a model of gene regulatory networks for anther differentiation and development. Our analysis provided insights into the extent of the function of key anther regulatory genes on the expression of hundreds of anther-expressed genes and uncovered components of putative cell-cell signaling and transcriptional networks.

Log in to add this item to your schedule

1 - The Pennsylvania State University, The Intercollege Graduate Degree Program in Plant Biology and Department of Biology
2 - The Pennsylvania State University, Department of Biology
3 - the Pennsylvania State University, College of Medicine, Department of Health Evaluation Sciences
4 - The Pennsylvania State University, Department of Biology, The Intercollege Graduate Degree of Plant Biology, The Huck Institutes of the Life Sciences, University Park, PA, 16802, USA

Differential expression
male sterile mutant

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P28014
Abstract ID:349

Copyright 2000-2007, Botanical Society of America. All rights