Sikora, Sergey , Lei, Huang , Xiangming, Fang , Wei-Wei, Zhang .
Digging into the Plant Proteome.
Avian polyclonal IgY (Immunoglobulin Yolk) antibodies have unique and advantageous features that allow highly-specific fractionation of complex protein mixtures. These unique features of IgY antibodies are extensively used in plasma proteome partitioning to remove highly-abundant plasma proteins. Immunoaffinity partitioning of plasma proteome has proven to be one of the most effective approaches for overcoming the wide dynamic range of protein concentration, as well as enabling the detection of low abundant proteins (LAP) in plasma/serum proteome. Similar challenges exist in plant biology. Rubisco, while being the key enzyme in photosynthetic carbon assimilation in green leafs, is the main obstacle in plant proteomics. It constitutes about 40% of the total protein mass in green leafs, thus interfering with proteomics studies such as LC-MS/MS and 2D-gel electrophoresis. We developed novel immunoaffinity system based on the IgY antibodies cross-linked to microbeads, which specifically removes Rubisco protein from plant extracts and other Rubisco-containing complex protein mixtures.
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1 - GenWay Biotech., Inc, 6777 Nancy Ridge Drive, San Diego, CA, 92121, USA
2 - GenWay Biotech
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM