| Abstract Detail
Genome Evolution Bocock, Philip [1], Dervinis, Chris [2], Davis, Jonh [2]. Evolution and Diversity of Invertase Genes in Populus trichocarpa. Invertase plays a key role in carbon utilization as it catalyzes the irreversible hydrolysis of sucrose into glucose and fructose. The invertase family in plants is composed of two sub-families thought to have distinct evolutionary origins. These sub-families are distinguished by their pH optima for activity: acid invertases apparently originated in eubacteria and are targeted to the cell wall and vacuole, while neutral/alkaline invertases apparently originated in cyanobacteria and function in the cytosol. The recently sequenced genome of Populus trichocharpa allows us to identify the genes encoding invertase in an economically important plant system, in order to understand both the evolutionary development of this family as well as its role in carbon allocation and partitioning in forest trees. Here we describe the identification of eight acid invertase genes; three of which belong to the vacuolar targeted group (PtVIN1-3), and five of which belong to the cell wall targeted group (PtCIN1-5). Similarly, we report the identification of 16 neutral/alkaline invertase genes (PtNIN1-16). An examination of the micro-syntenic regions surrounding the poplar invertase genes reveals extensive colinearity with Arabidopsis invertases. Expression analysis using both whole genome microarrays as well as quantitative RT-PCR reveals a general conservation of expression profiles of genes encoding acid and neutral invertase in response to auxin, nitrogen, and wounding treatments, and during development. We also find evidence for expression of a novel intronless vacuolar invertase (PtVIN1), which apparently arose through reverse transcription of a processed PtVIN2 transcript that subsequently inserted in the genome. To our knowledge this is the first intronless invertase found in plants. Log in to add this item to your schedule
1 - University of Florida, School of Forest Resources and Conservation, Newins-Ziegler, PO BOX 110410, Gainesville, FL, 32611, USA 2 - University of Florida, School of Forest Resources and Conservation
Keywords: Populus Invertase Colinearity.
Presentation Type: Plant Biology Abstract Session: P Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton Date: Sunday, July 8th, 2007 Time: 8:00 AM Number: P40005 Abstract ID:329 |