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Abstract Detail


Genomics / Proteomics

Ansari, M. Javed [1], Sindh, Kuldeep [2], Chhuneja, Parveen [2], Dhaliwal, H.S [1].

Isolation and characterization of brittle culm mutants of diploid wheat (Triticum monococcum) affecting mechanical strength of wheat tissues by altering the composition and structure of secondary cell walls.

Three EMS induced mutants in Triticum monococcum mbcl–12, mbcl -13 and mbcl -15, exhibit dramatically reduced tissue mechanical strength. Reduction in mechanical strength in the stalk tissue was highly correlated with a reduction in the amount of cellulose and an uneven deposition of secondary cell wall material in the subepidermal and perivascular sclerenchyma fibers. Cell wall accounted for two-third of the observed reduction in dry matter content per unit length of the mutant stalk in comparison to the wild type stalk, no compensation by lignin and cell wall matrix for reduced cellulose amount was observed. In these types of cells, mutations cause not only a reduction in cell wall thickness and cellulose content but also an increase in lignin level, suggesting that mbcl–12, mbcl -13 and mbcl –15 mutants reduce mechanical strength of Triticum monococcum tissues. Future activities will be focused on mapping, tagging and cloning of the brittle mutants using appropriate mapping population by anchored microsatellite markers.


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1 - Indian Institute Of Technology Roorkee, Department of Biotechnology, Roorkee, Uttranchal, 247667, India
2 - Punjab Agricultural University, Department of Plant Breeding, Genetics & Biotechnology, Ludhiana, Punjab, 141004, India

Keywords:
Btittle Mutants
Cellulose.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P72004
Abstract ID:30


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