| Abstract Detail
Secondary Metabolism Son, Seok Young [1], Rhee, Hong Soon [1], Cho, Hwa-Young [2], Park, Jeong Jin [1], Lee, Song Eun [3], Park, Jong moon [1]. The study of coumarins production after elicitation in Angelica gigas suspension culture. Industrial application of plant cell system is limited by low yields of target compounds and high cost of cell culture system, for example long culture and bioreactor. Many researchers have tried to overcome these limitations by optimization of plant cell systems and molecular biotechnology. But characterization of many medicinal plants was not still revealed. To enhance secondary metabolite productivity, characterization of medicinal plant must be investigated above all. Analysis of secondary metabolite and gene expression level related with biosynthetic pathway are very important.
Angelica gigas Nakai produces a variety of coumarins of pharmaceutical importance, including the decursin, decursinol angelate, nodakenin and ¥â-sitosterol. Angelica gigias has been used as a traditional Korean herbal medicine which has novel cancer chemotherapeutic compounds. Biosynthetic pathways of coumarin derivatives are associated with phenylalanine metabolism containing phenylalanine ammonia-lyase.
Elicitor treatment is a good tool for increasing productivity of secondary metabolites and revealing unexpected new secondary metabolites. We activated all secondary metabolisms by the addition of biotic elicitors to suspended cultured cells and compared secondary metabolites between control (unelicited) cells and elicited cells. Elicitor-treated cells showed higher productivity of coumarin and coumarin derivatives than untreated cells. Also Elicitor-treated cells produced secondary metabolites which are not shown in unelicited cell by opening other secondary metabolism. We investigated major relationship between profiles of metabolite production and expression level of phenylalanine ammonia-lyase (PAL). It gave us important information on characterization of traditional Korean medicinal plant in coumarin production pathway.
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1 - POSTECH, Department of Chemical Engineering, San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk, 790, Korea
2 - POSTECH, School of Environmental Science and Engineering, 416 SEE Hyojadong, Pohang, 790-784, KOREA
3 - POSTECH, Department of Chemical Engineering
Keywords:
Elicitation
Angelica gigas.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P20014
Abstract ID:277 |
