Lee, Sang-Choon , Koh, Serry , Kim, Min-Kyung , Choe, Sunghwa , An, Gynheung , Kim, Seong-Ryong .
OsGSK1, an orthologue of Arabidopsis BIN2, enhances tolerance to various abiotic stresses in transgenic rice.
T-DNA-tagged rice plants were screened under cold- and salt-stress conditions to determine the genes involved in the molecular mechanism for their abiotic-stress response. Line 0-165-65 was identified as a salt-responsive line. The gene responsible for this GUS-positive phenotype was revealed by inverse PCR as OsGSK1 (Oryza sativa glycogen synthase kinase3-like gene 1), a member of the plant GSK3/SHAGGY-like protein kinase genes and an orthologue of the Arabidopsis brassinosteroid insensitive 2 (BIN2), AtSK21. Northern blot analysis showed that OsGSK1 was most highly detected in the developing panicles, suggesting that its expression is organ-specific. Knockout (KO) mutants of OsGSK1 showed enhanced tolerance to cold, salt, heat, and drought stresses when compared with non-transgenic segregants (NT). These KO mutants also had large spikelets and long awns. Overexpression of the full-length OsGSK1 led to a stunted growth phenotype similar to the one observed with the gain-of-function BIN/AtSK21 mutant. This suggests that OsGSK1 might be a functional rice orthologue (OsBIN2) that serves as a negative regulator of brassinosteroid (BR)-signaling. Therefore, we propose that stress-responsive OsGSK1 may have physiological roles in stress signal-transduction pathways and floral developmental processes.
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1 - Sogang University, Department of Life Science
2 - Seoul National University, Department of Biological Sciences
3 - Pohang University of Science and Technology
4 - Sogang University, Department of Life Science, Mapo-gu, Sinsu-dong 1, Seoul, 121-742, Korea
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM