Thakare, Dhiraj R , Vajinder, Kumar , Jain, Pradeep K , Chikhale, Nandkishor J , Bhat, S. R. , Srinivasan, R .
Cloning and Characterization of an Anther Specific Promoter from Arabidopsis thaliana.
We here identified an Arabidopsis mutant line, anth85 expressing β-glucuronidase (GUS) specifically in the tapetal layer and microspores of developing anthers from a T-DNA tagged population carrying a promoter less GUS gene. This mutant carries a single T-DNA insertion in the upstream region of PER18 (AtPRX18, At2g24800) encoding a putative peroxidase. The transcript analysis of PER18 in wild type plants revealed maximal expression in the floral organs and very low level of expression in the roots and young seedlings. The homozygous plants for the mutation do not express the gene. The knockout of the gene in homozygous lines did not reveal any discernable change in phenotype. The 1.291kb 5′- regulatory region of the gene cloned in the binary vector driving reporter gene GUS in transgenic plants showed expression in inflorescence including anthers locules, anther filaments, sepals, petals, pedicel and peduncle. The 5′-UTR region of the gene was identified by 5′- RACE. A series of 5′- and 3′-deletions in the upstream region of PER18 were generated. The analysis of these upstream sequences has led to the identification of a 667 bp fragment (-667 to -1) of the peroxidase promoter that regulates GUS expression specifically in the tapetal layer of developing anthers.
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1 - University of Kentucky, Department of Plant and Soil Sciences, 301C Plant Science Building, University of Kentucky, Lexington, KY, 40546-0312, USA
2 - Indian Agricultural Research Institute, National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, 110012, India
3 - S.G.B. Amravati University, Department of Biotechnology, Amravati, MS, 444602, india
anther specific promoter.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM