Rehman, Riaz , zia, Muhammad , Chaudhary, Fayyaz .
Tissue culture studies of Caralluma tuberculata: an endangered plant.
Caralluma tuberculata, an endangered plant belongs to family Ascalpadaceae. Ethanobotanically this plant is used to cure diabetes and to control fat accumulation. Callogenesis and organogenesis protocols have been established. 2,4-D along with lower concentration of BAP in MS medium was found effective for callogenesis. Callus induction was observed after 8-10 weeks of placing the explant and cultures were fully developed in 16 weeks. Organogenesis was more complicated through callus and 3-4 shoots were observed at few combinations of plant growth regulators. Addition of TDZ along with Kin and 2,4-D was found effective for shooting response. Multiple shooting (18-20) response was observed through shoot tip explant and plant body section. Embryo formation was observed after six week of culture. TDZ alone or with very low concentration of GA3 was found to be effective for the multiplication and proliferation of shoots. Use of tissue culture technique can play effective role in saving this endangered specie of medicinal importance.
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1 - quaid-i-azam university, Departement of Biotechnology, Islamabad, 44000, Pakistan
2 - quaid-i-azam university, Departement of Biotechnology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM