Cytoskeleton Structure and Dynamics
Lucas, Jessica R , Sack, Fred D .
Microtubule arrays at the cytokinesis-interphase transition.
Plant microtubule arrays undergo major changes during the cell cycle, but some stage transitions are not well defined. During cytokinesis, most microtubules are associated with the phragmoplast. But after cytokinesis, during interphase, microtubules are primarily found in the cell cortex. At the transition from cytokinesis to interphase, existing data suggest that the first interphase microtubules form at the nuclear surface in suspension cells. But relatively little is known about how the cortical array is initiated in cells in tissues.
To observe how microtubules repopulate the cell cortex late in cytokinesis, we visualized microtubules in living Arabidopsis leaf epidermal cells harboring a constitutively expressed alpha-tubulin-GFP construct. The fine-staging of cells late in cytokinesis was facilitated by the predictable polarity of cytokinesis in this tissue. Our results indicate that microtubule nucleation in the cell cortex plays a significant role very early in the cytokinesis-G1 transition.
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1 - University of British Columbia, Botany, 3529-6270 University Blvd, Vancouver, BC, V6T 1Z4, Canada
2 - University of British Columbia, Botany
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM