| Abstract Detail
Reproductive Development Jones-Rhoades, Matthew [1], Borevitz, Justin [2], Preuss, Daphne [3]. Genome-Wide Expression Profiling of the Arabidopsis Female Gametophyte Identifies Families of Small, Secreted Proteins. The female gametophyte of flowering plants, the embryo sac, develops within the diploid (sporophytic) tissue of the ovule. While embryo sac expressed genes are known to be required at multiple stages of the fertilization process, the set of embryo sac expressed genes has remained poorly defined. In particular, the set of genes responsible for mediating intracellular communication between the embryo sac and the male gametophyte, the pollen grain, is unknown. We used high throughput cDNA sequencing and whole genome tiling arrays to compare gene expression in wild type ovules to that in dif1 ovules, which entirely lack embryo sacs, and myb98 ovules, which are impaired in pollen tube attraction. We identified nearly 400 genes that are down regulated in dif1 ovules. 78% of these embryo sac dependent genes were predicted to encode for secreted proteins, and 60% belonged to multigenic families. Our results define a large number of candidate extracellular signaling molecules that may act during embryo sac development or fertilization; less than half of these are represented on the widely used ATH1 expression array. In particular, we found that 37 out of 40 genes encoding Domain of Unknown Function 784 (DUF784) domains require the synergid specific transcription factor MYB98 for expression. Several DUF784 genes were transcribed in synergid cells of the embryo sac, implicating the DUF784 gene family in mediating late stages of embryo sac development or interactions with pollen tubes. The co-expression of highly similar proteins suggests a high degree of functional redundancy amongst embryo sac genes.
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1 - University of Chicago, Molecular Genetics and Cell Biology, w519
929 E. 57th Street, 929 E. 57th Street, Chicago, IL, 60637, United States 2 - University of Chicago, Evolution and Ecology 3 - University of Chicago, Molecular genetics and cell biology
Keywords: Embryo sac gene expression Tiling arrays DUF784.
Presentation Type: Plant Biology Abstract Session: P Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton Date: Sunday, July 8th, 2007 Time: 8:00 AM Number: P28064 Abstract ID:2704 |