Oikawa, Ai , Rahman, Abidur , Kidou, Shin-ichiro .
Virus-induced gene silencing of P23k in barley leaf reveals morphological changes involved in secondary wall formation.
P23k is a monocot-unique protein that is highly expressed in the scutellum of germinating barley seed. Our previous expression analyses suggested that P23k is involved in sugar translocation and/or sugar metabolism. However, the role of P23k in barley physiology remains unclear. Here, to elucidate its physiological function, we performed BSMV-based virus-induced gene silencing (VIGS) of P23k in barley leaves. Expression and localization analyses of P23k mRNA in barley leaves showed up-regulation of P23k transcript with increased photosynthetic activity and the localization of these transcripts to the vascular bundles and sclerenchyma, where secondary wall formation is most active. VIGS of the P23k gene led to abnormal leaf development, asymmetric orientation of main veins and cracked leaf edges caused by mechanical weakness. In addition, histochemical analyses indicated that the P23k distribution in leaves coincides with the distribution of cell wall polysaccharides. Considering these results together, we propose that P23k is involved in the synthesis of cell wall polysaccharides and contributes to secondary wall formation in barley leaves.
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1 - Iwate university, Cryobiosystem Research Center, Faculty of Agriculture Iwate University, Ueda 3-18-8, Morioka, Iwate, 020-8550, Japan
2 - Iwate University, Cryobiosystem Research Center
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM