Panthee, Dilip , Yuan, Joshua S. , Wright, David , Marois, James , Stewart Jr., C. Neal .
Differential expression of genes in soybean in response to Asian soybean rust (Phakopsora pachyrhizi Sydow) is specific to the soybean growth stage.
Understanding plant host response to the pathogen at the molecular level under different environmental or growth stages is crucial for developing a resistant plant variety. The main objective of this study was to perform a global transcriptome profiling of P. pachyrhizi -exposed soybean from three distinct developmental growth stages using whole genome Affymetrix microarrays of soybean. Three week-old soybean cv. 5601T and Glycine tomentella plants were inoculated with P. pachyrhizi and leaf samples were collected after 72 hours of inoculation. In contrast to only 25 differentially expressed genes at V2 growth stage, 5102 genes were differentially expressed at the V4 growth stage, of which 2523 were upregulated, and 722 were found to be expressed differentially at R1 growth stage, of which 333 were upregulated. There were 339 genes in common between V4 and R1 growth stage. A large difference in number of differentially expressed genes between the growth stages indicates that the gene expression is growth-stage-specific. Most of the differentially expressed genes were defense and stress related but there were a large number of unknown genes, which might potentially be ASR specific. We verified at least 20 genes from V4 and R1, and five from V2 growth stages by real-time RT-PCR for differential expression. This information may be useful to focus the microarray studies on a particular growth stage for gene discovery purpose.
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1 - University of Tennessee, Plant Sciences
2 - University ofFlorida, North Florida Agriculture Research and Education Center
3 - University of Florida, North Florida Agriculture Research and Education Center
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM