Unable to connect to database - 23:50:41 Unable to connect to database - 23:50:41 SQL Statement is null or not a SELECT - 23:50:41 SQL Statement is null or not a DELETE - 23:50:41 Botany & Plant Biology 2007 - Abstract Search
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Abstract Detail


Metabolism

Damari-Weissler, Hila [1], Ginzburg, Alexandra [1], Krassovskaya, Inga [2], Weber, Andreas P.M. [2], Granot, David [3].

Spinach SoHXK1 is a mitochondria-associated hexokinase.

Hexokinase, a hexose-phosphorylating enzyme, has emerged as a central enzyme in sugar-sensing processes. A few HXK isozymes have been identified in various plant species. These isozymes have been classified into two major groups; plastidic (type A) isozymes located in the plastid stroma and those containing a membrane anchor domain (type B) located mainly adjacent to the mitochondria, but also found in the nucleus. Of all the hexokinases that have been characterized to date, the only exception to this rule is a spinach type-B HXK (SoHXK1) that, by means of subcellular fractionation, has been localized to the outer membrane of plastids. However, SoHXK1 has a membrane anchor domain that is almost identical to that of the other type B HXKs. To determine the localization of SoHXK1 enzyme by other means, we expressed SoHXK1::GFP fusion protein in tobacco and Arabidopsis protoplasts and compared its localization with that of the Arabidopsis AtHXK1::GFP fusion protein that shares a similar N-terminal membrane anchor domain. SoHXK1::GFP is localized adjacent to the mitochondria, similar to AtHXK1::GFP and all other previously examined type B HXKs. Proteomic analysis had previously identified AtHXK1 on the outside of the mitochondrial membrane. We, therefore, suggest that SoHXK1 enzyme is located adjacent to the mitochondria like the other type B HXKs that share the same N-terminal membrane anchor domain.


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1 - Agricultural Research Organization, The Volcani Center, Plant Sciences
2 - Michigan State University, Plant Biology
3 - Agricultural Research Organization, The Volcani Center, Plant Sciences, P.O. Box 6, Bet-Dagan, 50250, Israel

Keywords:
Subcellular localization
Hexokinase
Hexose phosphorylation
GFP.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P19046
Abstract ID:2656


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