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Abstract Detail


Recent Topics Posters

Posto, Amanda L. [1], Jackson, Martina A. [1], Moyle, Leonie C. [1].

Genetic basis of postmating, prezygotic reproductive isolation and the developmental timing of male siring success.

Genetic barriers that lead to biological speciation can be investigated by understanding components responsible for reproductive isolation between species. Very little is currently understood regarding the genetic basis of failed male-female interactions in interspecific crosses even in model evolutionary systems. Using a population of near isogenic lines (NILs), we are investigating the genetic basis and behavior of prezygotic reproductive barriers that may act after pollination and prior to fertilization in Solanum. Each NIL contains a single introgression of a wild tomato species, S. pennellii, in the genetic background of the domesticated tomato, S. lycopersicon. We fertilized genetically uniform S. lycopersicon maternal plants with pollen from each NIL. By evaluating the success of each hybrid NIL genotype to successfully fertilize and sire seeds we can identify chromosomal regions (QTL) involved in prezygotic isolation due to dysfunctional signaling between male and female parents. We are also using staining techniques to examine the reproductive stage at which dysfunctional signaling occurs to pinpoint the precise stage at which NIL genotypes have failed during fertilization. Our results indicate that many failures to fertilize appear to be genotype specific and several regions of the tomato genome may contain complex male sterility QTL.


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1 - Indiana University, Department of Biology, Jordan Hall 142, 1001 E. 3rd St., Bloomington, IN, 47405, USA

Keywords:
reproductive isolation
Speciation
Solanum
tomato
NIL
QTL.

Presentation Type: Recent Topics Poster
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P79023
Abstract ID:2631


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