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Abstract Detail


Temperature Responses

Momonoki, Yoshie S. [1], Yamamoto, Kosuke [2], Oguri, Suguru [2], Okubo, Minoru [2].

Immunological Detection of Maize Acetylcholinesterase after Heat Stress.

The ACh-mediated system consisting of acetylcholine (ACh), acetylcholine receptor (AChR) and acetylcholinesterase (AChE) was recognized as a neuronal transmission system in animals. Both ACh and AChE activity have been widely recognized in plants. To clarify function of ACh-mediated system in plants, we have been investigated function of AChE from maize using histochemical and biochemical techniques. The maize AChE activity was mainly localized in endodermis between the stele and cortex in coleoptile node. Significant response of AChE activity was detected in endodermis in gravity- and heat-stressed maize seedlings. Thus, the maize AChE might respond to environmental stresses, and involve in solute distribution. We recently identified novel enzyme family of plant AChE (E.C. 3.1.1.7) that is distinct from animal AChE family, based on the AChE purified from maize. In the present work, we prepared a polyclonal antibody against maize AChE overexpressed in E. coli. The antibody was purified by antigen-affinity chromatography, and we investigated immunological detection of AChE in maize seedlings after heat stress. The maize AChE was mainly detected in coleoptile, coleoptile node and seed using immunoblot analysis. Furthermore, amount of maize AChE was increased following heat stress. These evidences demonstrate that maize AChE respond to heat stress.
* This work was supported by Japan Space Forum.


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1 - Tokyo University of Agriculture, Faculty of Bioindustry, 196 Yasaka, Abashiri, Hokkaido, 0992493, Japan
2 - Tokyo University of Agriculture, Faculty of Bioindustry

Keywords:
Acetylcholinesterase
heat stress
Immunolocalization.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P08007
Abstract ID:262


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