Park, Jong-In , Endo, Makoto , Kazama, Tomohiko , Hakozaki, Hirokazu , Takada, Yoshinobu , Kawagishi-Kobayashi, Makiko , Watanabe, Masao .
Functional analysis of the novel anther specific gene, AtPLIM2a, encoding LIM-domain proteins in Arabidopsis.
LIM proteins are important eukaryotic developmental regulators characterized by the presence of one or several double zinc finger motifs, the LIM domains, that mediates the protein-protein interactions in the transcription factors, signaling, and cytoskeleton-associated proteins. To date several LIM-domain proteins have been described in plants including sunflower, tobacco, and Arabidopsis. They contain two structurally very similar LIM domains separated by a spacer, and belong to the cysteine-rich protein (CRP) group of LIM proteins. Members of the mammalian CRP proteins have a joint localization in the nucleus and along the actin cytoskeleton, probably reflecting dual roles. In all higher plants, there appear to be two isoforms, one (PLIM) specific to pollen grains, and the other (WLIM) expressed in all other tissues. We identified five novel genes (AtPLIM2a, AtW/PLIM, AtWLIM2a, AtRLIM3a, AtRLIM3b), whose putative proteins contained one or two LIM domains, from Arabidopsis public database. From RT-PCR experiment, AtPLIM2a was specifically expressed in reproductive organ. Furthermore, the promoter-GUS and RNA in situ hybridization analysis of AtPLIM2a were specifically expressed in the tapetal tissues and the mature pollen grains. Also, fluorescence signal of the AtPLIM2a-GFP fusion protein, whose artificial gene construct was driven by the AtPLIM2a promoter, were observed at cytoplasm and vegetative nucleus in mature pollen grains. These date suggest that AtPLIM2a involves in the pollen development and pollen tube growth. Now, we are examining the function of the AtPLIM2a gene in the pollen tube growth and other reproductive characters.
Log in to add this item to your schedule
1 - Tohoku University, Graduate School of Life Sciences, 2-1-1 Katahira Aoba-ku, Sendai, 980-8577, Japan
2 - National Institute of Crop Science, Laboratory of Biotechnology
3 - Tohoku University, Graduate School of Life Sciences
In situ hybridization
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM