Education & Outreach
Tsay, Hsin-Sheng , Chen, Chin-Fun , Chen, Chien-Fu .
Activation tagging approach in a medical fungi, Antrodia cinnamomea.
In order to introduce concepts about activation tagging and Agrobacterium-based transformation, we used Antrodia cinnamomea, a Taiwanese medical fungi, to develop a laboratory exercise. Agrobacterium tumefaciens strain EHA105 harboring a standard binary vector pTAG-8 was used to generate transgenic mycelia population. More than 50 transgenic lines with normal and abnormal phenotypes were obtained. After HPLC and phenol-sulfuric acid analysis, the transgenic lines showed different compound profiles. Through this laboratory exercise, students learned not only knowledge of biochemical analysis, but also molecular biology techniques.
Log in to add this item to your schedule
1 - Chaoyang University of Technology, Graduate Institute of Biotechnology
2 - Chaoyang University of Technology, Graduate Institute of Biotechnology, 168, Gifong E. Rd., Wufong Township, Taichung County, TAIWAN, 41349, TAIWAN
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM