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Abstract Detail


Emerging Technologies

Okumoto, Sakiko [1], Chaudhuri, Bhavna [2], Frommer, Wolf-Bernd [2].

In vivo metabolite flux measurement in plants.

Plants need to sense external and internal stimuli, such as light, nutrition availability and nutrition requirement from different organs, and allocate their resources accordingly. Among macronutrients, carbon and nitrogen are the most required species in terms of quantities. Adjusting the C/N balance is essential for the growth and development of plants. In addition, many of C and N metabolites can act as signaling molecules. Indeed, about half of the Arabidopsis transcriptome is regulated by C, N or CN interactions (Gutierrez et al. 2007).
In order to understand carbon and nitrogen sensing and transport in plants, we need to be able to trace metabolite levels in higher spatial and temporal resolution. To meet such a requirement, we developed protein-based biosensors using Fluorescent Resonance Energy Transfer (FRET). By taking advantage of the bacterial periplasmic binding protein family, we were able to create metabolite sensors for many C and N metabolites, including glucose (Fehr et al. 2003), and glutamate (Okumoto et al. 2005), that play roles in metabolism and/or signaling. Results from Arabidopsis plants expressing such sensors suggest that these sensors can detect metabolite flux in cellular resolution, and also revealed that metabolism can be drastically different between different organs (Deuschle et al. 2006 Plant Cell, Okumoto unpublished result). We will introduce a new technique for the analysis of in vivo fluxes in plants and discuss our latest results.
Deuschle K, Chaudhuri B, Okumoto S, Lager I, Lalonde S, Frommer WB. (2006) Plant Cell 18(9):2314-25
Fehr M, Lalonde S, Lager I, Wolff MW, Frommer WB. (2003) J Biol Chem 278(21):19127-33
Gutierrez RA, Lejay LV, Dean A, Chiaromonte F, Shasha DE, Coruzzi GM. (2007) Genome Biol 8(1):R7
Okumoto S, Looger LL, Micheva KD, Reimer RJ, Smith SJ, Frommer WB (2005) Proc Natl Acad Sci USA 102: 8740-8745


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1 - Carnegie Institution of Washington, Plant Biology, 260 Panama St., Stanford, CA, 94305, USA
2 - Carnegie Institution of Washington, Plant Biology

Keywords:
biosensor
FRET
glucose
glutamate.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P44022
Abstract ID:2578


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