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Abstract Detail


Plant-Pathogen Interactions

Wang, Aiming [1], Raghupathy, Mohan [1], Huang, Tyng-Shyan [1], Griffiths, Jonathan [1], Yi, Jinxin [1], Gagarinova, Alla [1], Chen, Ling [1].

Virus-Plant Interactions: Molecular Identification of Host Genes Associated with Potyvirus Infection.

The development of novel antiviral strategies requires a better understanding of the complex molecular interplay between the host plant and the invading virus. Viruses encode only a very limited set of proteins, and thus largely depend on host gene products to fulfill their translation, replication and infection. In the past few years, four genomics approaches, i.e., expression sequence tags (EST), microarray (both oligoarray and cDNA array), cDNA-AFLP, and the yeast-two hybrid system, have been employed in our lab to study gene expression networks in peach (Prunus persica), soybean (Glycine max), Arabidopsis thaliana, and Nicotiana benthamiana in response to infection of two potyviruses, Plum pox virus (PPV) and Soybean mosaic virus (SMV). In the EST project, over 10,000 ESTs derived from PPV-infected and PPV-free peach were sequenced, and a comparative analysis of theses ESTs in the two different tissues was carried out as a discovery base for genes associated with PPV infection in peach. Microarray analysis using the Arabidopsis Affymetrix ATH1 chips (representing the entire genome of Arabidopsis) revealed about 3000 genes in Arabidopsis plants whose expression was either induced or suppressed by PPV. Soybean gene chips spotted with 18,000 cDNAs were used to monitor gene expression changes in SMV-infected soybean, leading to the discovery of 500 genes that were differentially regulated over the course of infection. In an endeavor to explore molecular events occurring at the early infection process, the Arabidopsis protoplasts were isolated and transfected with a PPV infectious clone. Approximately 550 differentially regulated genes were identified. In the cDNA-AFLP project, 158 differentially expressed cDNA fragments from PPV-infected N. benthamiana, were isolated and sequenced. Finally, the yeast-two hybrid assay screened out 215 peach proteins that interact with the PPV NIa-VPg and NIb proteins. Through these studies, a database containing over 4000 genes associated with SMV and PPV infection has been constructed. These data help largely advance our knowledge of virus-plant interactions. Further molecular and functional characterization of these candidate genes will provide target genes for the control of these viruses.


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1 - Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford Street, London, Ontario, N5V 4T3, Canada

Keywords:
Soybean mosaic virus
Plum pox virus
plant-virus interaction
Soybean
Arabidopsis thaliana
Peach
Nicotiana benthamiana
microarray
EST
cDNA-AFLP
Yeast-two hybrid.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P15015
Abstract ID:255


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