Ogita, Shinjiro , Himeno, Arisa , Kato, Yasuo .
Metabolic analysis of maltol and maltol glycoside in Cercidiphyllum japonicum.
Maltol (3-hydroxy-2-methyl-4-pyrone) is known one of major plant-derived aromatic substance that is widely used for spice and food additive. Cercidiphyllum japonicum is known as a hyper accumulator of maltol and its glycoside. We first determined change in levels of these substances in leaves of C. japonicum. Leaves, i.e. green, yellow, and brown in colour, were collected from late August to early November in 2006 at Toyama Prefecture. They were extracted and purified by appropriate methods and were applied to HPLC analysis. We found that the accumulation pattern of maltol was correlated with leaf senescence. Yellow-brownish old/fallen leaves contained a large amount of maltol while young green leaves mainly contained its glucoside form. Concentrations of endogenous amino acids and carbohydrates in the samples were also measured. Furthermore, callus culture was established for detailed analysis of accumulation pattern of maltol and its glycoside in vitro. Callus was initiated from in vitro germinated seedlings on a modified Murashige and Skoog medium containing 2 uM TDZ. By using the callus culture system, effects of carbohydrates, phytohormones, pH, temperature, and photoperiod on callus growth were investigated to identify the biosynthetic rule of maltol. Artificial senescence of in vitro grown tissues could be regulated by carbohydrates graduation and pH adjustment. Maltol accumulation was strongly enhanced in a suitable condition tested.
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1 - Toyama Prefectural University, Department of Biotechnology, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan
2 - Toyama Prefectural University
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM