| Abstract Detail
Secondary Metabolism Behbahani, Mandana [1]. Optimization of growth and lycopene production in callus and cell suspension cultures of Barringtonia racemosa. Abstract Leaf explants of B.racemosa were placed on Murashige and Skoog (MS), Woody Plant Medium (WPM) and B5 media, containing growth regulators to determine the optimum conditions for callus initiation and maintenance. The explants were cultured either in dark or in light (2 Klux 16 h/day) at 25+1°C. Friable, fast growing callus was evident after 5 weeks on WPM+ 2 mg/L (w/v) of 2,4-D. Callus initiation occurred sooner on WPM than on MS and B5 media. Calli cultures derived from the leaves of B.racemosa were selected on the basis of colour into green callus and pale yellow callus in the light and dark conditions respectively and it was suggested different levels of lycopene accumulation. An improved reversed phase high performance liquid chromatographic method provided selective determination of the lycopene content of these samples. The lycopene concentration in green callus was higher than that of the pale yellow callus. Analysis of growth and lycopene content of callus cultures and cell suspension cultures demonstrated that the production of lycopene was growth-dependent in both callus and cell suspension culture.
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1 - malaysia, selangor, serdang, UPM
Keywords:
none specified
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P20050
Abstract ID:2480 |
