Abstract Detail
Protein Modification and Turnover Ewan, Richard [1], Sadanandom, Ari [2]. The tobacco Ubiquitin-specific protease NtCDD1 positively regulates cell death during plant disease resistance. The hypersensitive cell death response (HR) and disease resistance are intimately connected and crucial in plant defence against pathogens. Although Resistance (R) proteins are known to be important players in pathogen recognition little is known of the molecular mechanisms downstream of this recognition event leading to hypersensitive cell death. The Ubiquitin-Proteasome system is a key mechanism controlling many signaling pathways and we recently showed that the E3 ubiquitin ligase AtPUB17 plays a crucial role during disease resistance specified by multiple R-genes. We have now identified a Ubiquitin-specific Protease (NtCDD1) that positively regulates Avr9/Cf9 elicited hypersensitive cell death in tobacco. We have demonstrated bona fide Ubiquitin-specific protease activity of NtCDD1 in vitro. We silenced NtCDD1 in N. benthamiana to demonstrate its central role in plant disease resistance against a range of fungal and viral pathogens. The target proteins deubiquitinated by NtCDD1 represent key regulators of the plant HR. This data represents a first example linking regulatory deubiquitination to disease resistance signaling in plants.
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1 - University of Glasgow, Plant Science, Arnott Lab, Bower Building, University Avenue, Glasgow, G12 8QQ, Scotland
2 - Plant Science, Biochemistry and Molecular Biology
Keywords:
ubiquitination
deubiquitination.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P37004
Abstract ID:248 | 