| Abstract Detail
Organelle Biology Kwon, KwangChul [1], Cho, Myeon Haeng [2]. The chloroplast-localized AtTerC gene product in Arabidopsis thaliana is required for the biogenesis of thylakoid membrane and for the chloroplast development. The early seedling development of plants depends on the chloroplast formation from proplastid, but this event is impossible without thylakoid biogenesis. An Arabidopsis thaliana gene, named AtTerC, which contains a domain similar to bacterial tellurite resistance C (TerC), is shown here involved in critical step required for the normal organization of prothylakoid into mature thylakoid stacks and ultimately for leaf development. AtTerC gene encodes an integral membrane protein localized in thylakoid of chloroplast, as shown by localization of a TerC:GFP fusion product in protoplast and by immunoblot analysis of sub-fractions of chloroplast. T-DNA insertional mutants displayed a pigment defective and seedling lethal phenotype in normal light condition. Transmission electron microscopy of etioplast and chloroplast revealed that the formation of prothylakoid and thylakoid membrane in T-DNA knockout mutant was severely impaired. Mutant chloroplasts were highly vacuolated, lacked internal membrane structures, and showed abnormal sub-organellar structures. The block of thylakoid membrane biogenesis of mutant seedlings caused the severely reduced accumulations of nuclear-encoded photosynthetic proteins, and many of anti-oxidant genes were up-regulated as a consequence of secondary effect of the mutation. These results suggest that the AtTerC protein plays a crucial role in prothylakoid membrane biogenesis and thylakoid formation in early seedling development. [This work was supported by The Second Phase of BK21 Program (YBRI) of Korea].
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1 - Yonsei univ., Biology, 134 Shinchon-dong, Seodaemun-gu, seoul, 120-749, Korea
2 - Yonsei univ., Biology
Keywords:
prothylakoid
thylakoid
Chloroplast
seedling lethal.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P18033
Abstract ID:2477 |
