| Abstract Detail
Protein Targeting and Vesicular Trafficking Lee, Young Nam [1], Nomura, Kinya [2], He, Sheng-Yang [2]. Characterization of subcellular localization and functions of AtMIN7 in Arabidopsis thaliana. We are testing the hypothesis that one function of type III effectors (TTEs) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) is to suppress defense-associated secretion processes in Arabidopsis thaliana. Our recent results showed that AtMIN7, a member of the Arabidopsis ARF-GEF family, is targeted and degraded by a TTE, HopM1. Knockout mutants of AtMIN7 showed enhanced susceptibility to ΔCEL mutant of Pst DC3000, which does not have a functional HopM1, indicating that AtMIN7-mediated trafficking process is important for the Arabidopsis defense. We are studying the subcellular localization of AtMIN7 using green fluorescence protein (GFP) tagging to AtMIN7 ORF followed by confocal microscopic observation.We are also searching for defense-related Arabidopsis proteins which may be delivered to their subcellular destinations through AtMIN7-mediated trafficking.
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1 - Michigan State University, PRL/ Plant BIology, #222 Plant BIology Building, Michigan State University, East Lansing, Michigan, 48824, USA
2 - Michigan State University, PRL
Keywords:
AtMIN7
confocal microscopy.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P22034
Abstract ID:2458 |
