Yeh, Ching-Hui , Lin, Ya-Ping , Chen, Ming-Tse .
Characterization of a chemical responsive element on promoter of rice class I small heat shock protein genes.
Using transient expression assays of the promoter activity by linking to GUS reporter gene, a possible azetidine (Aze)-responsive element (AZRE; GTCCTGGACG) on the promoters of rice class I small heat shock proteins (sHSP-CIs; Oshsp17.3 and Oshsp18.0) is found to be capable of directing expression during Aze treatment. However, Aze-induced accumulation of Oshsp17.3 is different from that of Oshsp18.0 in the presence of cycloheximide. Analysis of GUS activity of Arabidopsis transgenic plants in response to stress induction indicated that GUS activity of p567(＋) (promoter of Oshsp18.0) transgenic plants and p567(－) (promoter of Oshsp17.3) transgenic plants increased under HS, Aze, arsenite, cadmium, and copper treatment. In contrast, GUS activity is not detected in Aze treated p567(－)ΔAZRE (promoter of Oshsp17.3 without AZRE) transgenic plants, although these transgenic plants showed low expression level of GUS activity under HS stress. Interestingly, truncation of AZRE did not affect GUS activity of transgenic plants under As, Cd, and Cu treatment. Furthermore, EMSA result indicates a specific factor involved in regulating Oshsp17.3 and Oshsp18.0 gene expression. All these results indicate that AZRE is not only specific for Aze responsiveness but also necessary for HS response on sHSP-CI genes.
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1 - Graduate Institute of Life Science, National Central University, Taoyuan, Taiwan, ROC
small heat shock proteins
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM