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Abstract Detail

Bio-Energy Crops

Marton, Laszlo [1], Czako, Mihaly [2], Pollock, Robert [3].

Mass propagation and improvement of Arundo donax a promising biomass energy crop.

Monoculture forming monocots are important components of different ecosystems and are often able to produce huge biomass, more than most cultivated plants.
Tissue culture is prerequisite for both large scale propagation for environmental biotechnology applications and genetic manipulations, such as mutant selection and genetic improvement by introduction of key transgenes which can significantly improve their utilization for remediation and increase their biomass quantity and quality. We have developed a universal cell culture initiation medium and procedure which allowed us to establish embryogenic cell cultures, micropropagation and genetic engineering protocols, which have successfully been used for a great number of species from a diverse group of monocot species (Cyperaceae, Juncaceae, Poaceae, and Typhaceae). Our technology has been fully explored with Arundo donax, down to the large scale application details, including storage, transportatation and deployment, as well as somaclonal breeding. The method comprised using immature inflorescences as explants and cultivating the tissue on a primary medium to produce totipotent embryogenic tissue culture, cultivating the embryogenic tissue on a secondary medium to produce regenerating cultures, then sustaining shoot multiplication and production of complete plantlets having roots and shoots on tertiary medium before acclimating the plantlets in soil.

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1 - University of South Carolina, Biological Sciences, 730 Sumter, Columbia, SC, 29208, USA
2 - University of South Carolina, Biological Sciences
3 - Southern Sun Biosystems Inc., Hodges, SC,

mass propagation
somaclonal breeding.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P03002
Abstract ID:244

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