Modeling and Computational Biology
Jung, Woosuk , Kang, Kyung-Ho , Chung, Ill-Min , Jeung, Ji-Ung , Kim, Min Jin , Heo, Hwa-Young , Park, Na-Young , Roh, Kwang-Soo .
Development and Application of Gene Specific Molecular markers based on Polymorphic Diversities of Promoter Region.
Developing molecular markers which are related with a specific traits provides a solid fundamental for breeding particularly if the target trait is a quantitative trait such as amylose and secondary metabolites contents of grains. Several molecular markers like RFLP, RAPD, SSR, AFLP and SNP marker have been used for crop breeding. The polymorphic patterns at the genome and transcriptome levels between heterologous counterpart lines are used for selectable markers if they are closely related with ceratin traits. However, still those relationships could not provide enough information to narrow down the target gene which is directly related with the trait, because most of those markers have been identified by chance and came from intergenic regions. In this study, we tried to identify several differently expressed genes between the parents showing counterpart pattern on target trait by the using microarray analysis and functional screening. After identifying differently expressed genes we extracted putative promoter region sequences from existing genome sequencing databases and designed primer sequences which will possibly produce an amplified DNA fragment by PCR. Among them we found several gene specific primers which can amplify promoter regions showed polymorphism between the parent lines. With this method, we can clones candidate gene which is related with quantitative traits such as rice blast resistance, amylose content and allelopathy.
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1 - Konkuk University, Applied Bio-Sciences, 1 Hwayang-dong, Kwangjin-gu, Seoul, Korea (R.O.K)
2 - National Institute Crop Sciences, Crop Genetics and Breeding
3 - Konkuk Universtiy, Applied Bio-Sciences
4 - Konkuk University, Applied Bio-Sciences
5 - National Institute Crop Sciences, Crop Functionality
6 - Keimyung University, Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM