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Verma, Anshu [1].

Purification and characterization of chitinase enzymes in edible mushrooms culture grown in different medium.

Mushrooms are fruiting bodies to the class Basidiomycetes some mushrooms are edible and some are non edible. Its low in calories, rich in proteins, minerals and vitamins. They have been recognized as the alternate source of good quality protein. Its lies between meat and vegetables. The role of extracellular enzymes is pivotal to the production of biomass. Chitin (linear poly β-1, 4-N-acetyl-D-glucosamine) is the second most abundant biopolymers on the earth. Fungi composed of chitin and chitinase are involved in morphogenesis. Total 12 species/strains/isolates were taken for the present study. They were collected for different area of Madhya Pradesh and Chattisgarh in India. Six various composition of media used for the growth of mushroom mycelium. After screening of media, suitable media was selected for growth of biomass. Experiments were conducted under both solid and liquid media. The result observed the maximum mycelial colony was found in Pleurotus florida in Potato dextrose agar media. The maximum biomass of fungus has been found in Pleurotus species from potato dextrose broth. The mycelium mat of P. florida has very thick, cottony growth and milky white and other fungus showed a creamy layer of mycelial mat.Mycelia of all the isolates of edible mushrooms was initially cottony white, which later turned to milky white on solod media. The activity of extracellular enzymes chitinase has been studied in various mushrooms. The overall maximum activity was almost same in Calocybe indica and Pleurotus florida. It was observed that there is positive association between biomass production and enzyme activity respectively. The molecular weight of the chitinase enzymes was calculated 36 kDa by gel filteration on sephedex G-200 in P. florida.


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1 - J.N.K.V.V. Jabalpur (M.P.) India, Biotechnology Centre, 610 Shakti Nagar, Jabalpur, Madhya Pradesh, 482001, India

Keywords:
enzymes
Chitinase
mushrooms
media
purification.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P46023
Abstract ID:2386


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