Growth and Vegetative Development
Lugassim-Ben Hamo, Maya , Zaccai, Michele .
Characterization of floral transition in lisianthus (Eustoma grandiflorum).
Lisianthus (Eustoma grandiflorum) is an ornamental plant originating from US. Temperature plays a major role in the control of lisianthus growth and flowering, depending on the developmental stage of the plants. Transition from the vegetative rosette to reproductive stage is inhibited by high temperature, while higher temperatures after stem elongation accelerates flowering. In Israel, lisianthus production is directed to winter and spring, when production in Europe is reduced and prices are high. In order to decrease the temperatures at planting (during late fall), shade nets are used. The objectives of the study were (1) investigate the effects of shade on lisianthus development, floral transition, flowering time and inflorescence quality; (2) analyze the soluble sugars and starch contents of the plants under the different shade treatments and (3) to establish the lisianthus meristem proteome before and after floral transition. Planting time, largely mediated by temperature, had a strong effect on floral transition, while shade regime significantly affected the plants yield and quality. Longest and heaviest shade treatments caused greatest delays in flowering and decreased yield. Starch content was significantly affected by shade treatments. Overall, these results demonstrated that growth and flowering of lisianthus may be manipulated through shade treatment. We used IPG Ready Strips (7 CM) MW 4-20% and SYPRO Ruby protein gel stain, in order to optimizing the two-dimensional polyacrylamide gel electrophoresis protocol for generate stage-specific primordia protein maps and isolate differentially-expressed proteins.
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1 - Ben-Gurion University of the Negev, Life-Sciences, Campus Bergmann, POB 356, Beer-Sheva, 84105, Israel
2 - Ben Gurion University of the Negev, Department of Life Sciences
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM