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Abstract Detail

Cytoskeleton Structure and Dynamics

Sattarzadeh, Amirali [1], Schmelzer, Elmon [2], Hanson, Maureen R. [3].

In vivo tagging and subcellular targeting of myosin XI isoforms of Arabidopsis thaliana.

Myosins are actin-based molecular motors that play fundamental roles in many forms of motility. Knowledge about structure and function of plant myosins is very limited. Major sources of information about higher plant myosin genes are the completed genome sequences of Arabidopsis thaliana and Oryza sativa. 17 myosin genes were identified in the Arabidopsis genome; 4 of them belong to class VIII and 13 to class XI; the rice genome encodes 14 myosin genes (2 class VIII and 12 class XI). Though the plant myosins appear to form a clade of their own, the available sequence data nevertheless suggest that they follow the domain pattern typical for all known myosins. Based on the published immunocytochemical data for class XI myosins and the possibility that they function like class V myosins in transport of cargo, one of the most attractive questions is which types of vesicle or organelle interact with which plant myosin. In this study a survey of the subcellular localization, the dynamic behavior, the transported cargoes and their potential binding site(s) in myosin tails was performed. The data show that specific binding sites for Golgi stacks/vesicles and peroxisomes, which are highly conserved among all A. thaliana class XI myosins, reside within the C-terminal part of class XI myosins. Co-localization of class XI myosins with mitochondria suggests that class XI myosins might be involved in mitochondrial transport in plant cells. Examination of the phenotype of 10 out of 13 class XI myosin gene homozygous knock-out lines revealed no drastic effects with respect to growth and development or pathogen defense. Altogether, these results demonstrate considerable functional redundancy among class XI myosins.

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1 - Cornell Uuniversity, Molecular Biology and Genetic, Biotechnology Building, Hanson Lab, Ithaca, NY, 14853, USA
2 - Max-Planck-Institute for Plant Breeding Research, Cologne, Germany, Central Microscopy
3 - Cornell Uuniversity, Dept. of Molecular Biology and Genetics

molecular motor

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P23013
Abstract ID:2362

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