Ebbs, Stephen , Milner, Matt , Spiller, Shawna , Kochian, Leon .
Unraveling heavy metal homeostasis in the Zn/Cd hyperaccumulator Thlaspi caerulescesns: membrane transport and microarray studies.
The Zn/Cd hyperaccumulating plant Thlaspi caerulescens has been a valuable model for the study of hyperaccumulation, hypertolerance, and ion homeostasis in plants, particularly since there are populations of this species that vary in their capacity to hyperaccumulate Cd. The studies here used two populations of T. caerulescens, Prayon and Ganges, in a series of membrane transport studies with excised leaf section in an effort to determine the contribution of cell wall binding, influx, and efflux to the Cd hyperaccumulation displayed by each population. Results from these studies demonstrated that the Ganges population displays a significantly greater rate of influx as compared to Prayon, but that efflux from leaf sections of Prayon was significantly greater than that for Ganges. In parallel, a microarray study was conducted using plants of Prayon and Ganges exposed to a high, sub-toxic concentration Cd. The genes up-regulated in each population in response to Cd are being identified and, along with the results from the previous study and other published works, used to construct a conceptual model to explain the difference in Cd hyperaccumulation by these two populations.
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1 - Southern Illinois University Carbondale, Department of Plant Biology, 420 Life Science II, 1125 Lincoln Drive, Carbondale, IL, 62901-6509, USA
2 - Cornell University, Section of Plant Biology, U.S. Plant, Soil, and Nutrition Laboratory, Tower Rd., Ithaca, NY, 14853, USA
3 - Southern Illinois University Carbondale, Plant Biology, 420 Life Science II, 1125 Lincoln Dr., Carbondale, IL, 62901-6509, USA
4 - U.S. Dept. of Agriculture, Agricultural Research Service, Tower Rd., Cornell University, Ithaca, NY, 14853, USA
metal ion homeostasis.
Presentation Type: Oral Paper:Papers for Sections
Location: Stevens 1/Hilton
Date: Wednesday, July 11th, 2007
Time: 2:15 PM