Dzianott, Aleksandra , Bujarski, Jozef .
Effects of Gene Knockouts that Function in the siRNA Biogenesis Support the Role of RNA Interference (RNAi) Pathway in the Brome Mosaic Virus RNA Recombinant Formation in Arabidopsis thaliana.
We have reported that a knock-out mutation in the SGS-2 (RDR6) RdRp gene reduced the fraction of nonhomologous chimeric RNA3-RNA1 recombinants in Brome mosaic bromovirus (BMV)-infected Arabidopsis plants. This suggested the role of the RNA interference (RNAi) pathway in recombinant formation. To further explore this possibility in this work we are testing the following gene-knockout Arabidopsis lines: sgs-1, sgs-3 (a novel plant-specific coiled-coil protein), and Ago-1 (a slicer in the RISC complex). SGS-3 and RDR6 function in the same pathway of trans-acting siRNA biogenesis whereas sgs-1 mutant impairs PTGS by an unknown effect. In addition we are testing the knockout lines in the DCL (Dicer-like) 1 through 4 genes. A control involves the rpa2-3 mutant that functions during TGS but does not affect PTGS-related siRNA synthesis. These studies revealed various effects on both the recombination frequency and the RNA3-RNA1 recombinant profiles, strengthening the notion that RNAi participated in the formation of BMV RNA recombinants.
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1 - Northern Illinois University, Biological Sciences
2 - Northern Illinois University, Biological Sciences, Plant Molecular Biology Center, Montgomery Hall, DeKalb, IL, 60115, USA
genetic RNA recombination
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM