Mechanisms of Gene Regulation
Zhou, Fujun , Roy, Bijoyita , von Arnim, Albrecht .
Defining the role of translation initiation factor eIF3h in Arabidopsis.
The regulation of translation initiation plays important roles in plant development and environmental stress responses. With twelve subunits, eIF3 is the largest of the eukaryotic initiation complex yet little is known about the structural and functional roles of individual subunits, in any organism. Arabidopsis insertion mutants that cause truncations of eIF3h gene show pleiotropic growth defects which, under certain growth conditions, include pin-formed inflorescences characteristic of aberrant auxin transport or response, gene expression assays indicate that eIF3h contributes to translation initiation of mRNAs harboring upstream open reading frames (uORF), which are a conserved feature of many mRNAs, including those of auxin response transcription factors. To further investigate the role of eIF3h in translation initiation, a broad spectrum of 5’ upstream leader sequences are being examined for their dependence on eIF3h in DNA and RNA-base expression assays. We are exploring the utility of an internal ribosome entry site as a reference reporter gene and the possible dependence of cap-independent translation initiation on eIF3h.
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1 - The Univesity of Tennessee, Graduate School of Genome Science and Technology, Knoxville, Tennessee, 37996, USA
2 - The University of Tennessee, Department of Biochemistry, Cellular and Molecular Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM