Celaya, Brandon , Campell, Thomas , Luesse, Darron , Galen, Candace , Hangarter, Roger , Liscum, Mannie .
HYPER-PHOTOTROPIC HYPOCOTYL mutants exhibit unusual effects on phototropin mediated processes in Arabidopsis.
Phototropins are blue-light photoreceptors shown to have key roles in processes such as phototropism, stomatal movement and chloroplast relocation. We employed a genetic approach to identify mutants that show aberrant phototropic responses. One of the mutants identified showed enhanced phototropic responsiveness and thus was named hyper-phototropic hypocotyl (hph). hph mutants also appear to have other enhanced phototropin-associated phenotypes. Our analysis to date indicates hph may be an allele of the PHOT1 gene itself, though we are continuing to confirm this result at the molecular level. Our genetic analyses indicate that the phot1-9hph hyper-responsive phenotypes arise only when the mutant allele is present with a wild-type allele, while the loss-of-function hypo-responsiveness arises when the phot1-9hph allele is in the homozyous state. We are attempting to recapitulate these phenotypes through transgenic plant generation. Based on analysis of hph and biophysical data from other research groups, phot1 is proposed to interact with a number of other proteins and possibly itself in order to facilitate phototropin-associated signaling. We have therefore also undertaken a yeast-two-hybrid approach to deduce key regions of phot1 necessary for known protein-protein interactions as well as addressing the question of phototropin-phototropin dimerization in vivo.
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1 - University of Missouri, Division of Biological Sciences, 302 Life Sciences Center, Columbia, MO, 65211, USA
2 - North Georgia College and State University, Biology
3 - Indiana University, Biology
4 - University of Missouri, Division of Biological Sciences
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM