Hartwig, Thomas , Obrdlik, Petr , Frommer, Wolf-Bernd , Schulz, Burkhard .
Protein-protein interaction of the immunophilin-like TWISTED DWARF1 with membrane transporters in Arabidopsis.
The immunophilin-like FK506-binding protein (FKBP) TWISTED DWARF1 (TWD1/FKBP42) is playing a critical role in morphogenesis and development in Arabidopsis. TWD1 has been shown to physically interact with various p-glycoprotein (PGP), ATP-binding cassette (ABC) transporters, including the plasma membrane localized AtPGP1 and AtPGP19, as well as vacuole localized ABC transporters AtMPR1 and AtMPR2. Mutant analysis of null mutations and expression of TWD1 together with AtPGP1 and AtPGP19 in yeast and HeLa cells support the role of TWD1 as regulator of auxin exporters AtPGP1 and AtPGP19. Therefore, polar auxin transport is affected in twd1 mutants. Null mutants of the AtFKBP42 gene are characterized by reduction of cell elongation which leads to dwarfism and disoriented cell growth, leading to the name twisted dwarf1 (twd1). Further interactions of the multi-domain immunophilin-like TWD1 with HSP90 and calmodulin have been shown in vitro, although the physiological function of these interactions is not clear yet. In order to better understand the dramatic pleiotropic phenotype which can not alone be explained by loss of AtPGP function, further tests to investigate additional interactions with membrane proteins have been started. We will present data from interaction testing with additional putative interactors employing the split ubiquitin system and in vitro protein-protein interactions.
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1 - Purdue University, Horticulture and Landscape Architecture, 625 Agricultural Mall Drive, West Lafayette, IN, 47907, USA
2 - IonGate Inc., Research
3 - Carnegie Institution, Department of Plant Biology
4 - Purdue University, Horticulture and Landscape Architecture
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM