Chen, Chien-teh , Jan, Fuh-Jyh .
virus induced gene silencing on tobacco and phytoremediation.
The aim of this research is to evaluate the possibility of using virus-induced gene silencing (VIGS) on tobacco to manipulate the expression of proline metabolism related genes and to remediate the heavy metals polluted soils. The fragment of proline metabolism related genes were constructed in TMV. After the inoculation of TMV for 4 weeks, the tobacco leaf disks were subject to the determination of drought induced proline accumulation. The results demonstrated that the inhibition of proline synthesis gene, P5CS, via VIGS decreased the amount of drought induced proline accumulation by 30 to 50%. The inhibition of proline synthesis gene, OAT, decreased the amount of drought induced proline accumulation by 10 to 30%. The inhibition of proline synthesis gene, P5CR, did not affect the accumulation of proline. The inhibition of proline catabolism gene, PDH, increased the accumulation of proline by 30 to 50%. After the inoculation of TMV for 4 weeks, the tobacco plants were subject to excess Cu treatment for 6 days. The results of Cu contents in tobacco plants showed the inhibition of proline metabolism related genes, either synthesis or catabolism, increased the Cu contents up to six folds in spite of the difference of proline levels.
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1 - National Taiwan University, Department of Agricultural Chemistry, Taipei, 106, Taiwan
2 - National Chung Hsing University, Department of Plant Pathology, Taichung, 40227, TAIWAN
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM