Walck, Aaron , Melmaiee, Kalpalatha , Abebe, Tilahun .
Response of the barley proteome to drought stress.
Drought is the major environmental factor that depresses crop yield. Drought affects all developmental stages of crops, the reproductive stage being the most sensitive. Depending on the duration and severity of the stress, shortage of water at the reproductive stage can cause irreversible yield loss due to low test weight and fewer seeds per plant. In cereals, moderate drought causes senescence and wilting of leaves. Under this condition, assimilates from photosynthetic organs of the spike can sustain grain development. However, if the drought is severe, availability of assimilates from the spike will be limited leading to reduced grain filling. Understanding gene expression in drought-stressed spikes can facilitate development of varieties that give better yield when available water is limited at the reproductive stage. Recently, we have started investigating gene expression patterns at the proteome level in the reproductive structures of barley. Barley plants were drought-stressed by withholding water for four to five days before grain filling. The spike was collected from stressed and non-stressed plants to isolate total proteins. Proteins were separated using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Over 600 protein spots were detected from drought-stressed and well-watered spike tissues. Matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry is being performed to determine identity of the proteins that accumulate in the spike during drought stress. Further proteome analysis is being performed to determine response of the proteome to drought under different developmental stages of the spike.
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1 - University of Northern Iowa, Department of Biology, 144 McCollum Science Hall, Cedar Falls, Iowa, 50614, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM