Basu, Manojit , Gonzalez-Carranza, Zinnia , Azam-Ali, sayed , Roberts, Jeremy .
A Novel Strategy to Dissect the Role of Hormones in the Regulation of Cell Separation in Arabidopsis thaliana.
Cell separation is an important process in the life cycle of plants, regulating abscission, dehiscence, root elongation and lateral root emergence. During abscission and lateral root development cell separation is brought about by the action of several hydrolytic enzymes; one of these has been identified as polygalacturonase (PG). In this study we have used three genes which are able to modify IAA biosynthesis and have been fused to the promoter of an Arabidopsis PG gene (At2g41850) for tissue specific expression in the floral AZ, the root pericycle and cortical cells. A second strategy has been used to study the effect of At2g41850-driven silencing of IAA response during abscission and lateral root emergence using the AXR3 gene. Interaction between ethylene, ABA, GA, and cytokinins has been investigated during cell separation. Delayed abscission and higher tissue break strength has been recorded in plants transformed with genes up-regulating IAA biosynthesis. The expression of the IAA manipulating genes under the control of the At2g41850 promoter has altered the root architecture and the impact of this on plant abiotic stress tolerance has been evaluated by measuring WUE and root growth under various levels of soil compaction.
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1 - University of Nottingham, Plant Sciences, School of Biosciences, SB Campus, College Road, Loughborough, Leics, LE12 5RD, UK
2 - University of Nottingham, Plant Sciences
3 - University of Nottingham, Agricultural and Environmental Sciences
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM